Relay assembly techniques have revolutionized molecular biology by providing innovative methods for assembling large and complex DNA constructs with high efficiency and accuracy. These techniques are especially crucial in synthetic biology, genetic engineering, and the development of recombinant DNA molecules, enabling the combination of multiple DNA fragments into one seamless construct. This article explores some of the most widely used relay assembly methods, highlighting their principles, applications, and advantages in modern genetic research.

The Concept of Relay Assembly Relay assembly refers to a set of techniques that facilitate the joining of multiple DNA fragments in a systematic manner, often through enzymatic reactions or recombination processes. The goal of these techniques is to simplify the process of constructing recombinant DNA molecules from smaller fragments, which can be a challenging task, especially when the fragments are large or numerous. Relay assembly techniques ensure that the DNA fragments are correctly oriented and connected, reducing the likelihood of errors during cloning. Golden Gate Assembly： A Popular Relay Assembly Method